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Research
Detection of Porcine Rotavirus using Photonic Crystal Biosensors
Ground water monitoring is an important issue in developing safe water detection for human and farm animals. A type of pig virus called rotavirus has the potential to kill pigs on the farming region in the irrigation water. In order to detect rotavirus in the water, samples have to be collected, concentrated, and examined using fluorescently labeled methods such Enzyme-Linked Immunosorbent Assay (ELISA), which can be very expensive. In this work, we detected specifically rotavirus binding to immobilized anti-rotavirus immunoglobulin G (IgG) on photonic crystal biosensor, with a signal comparable or even better than the commercialized ELISA kit, and it is the first time label-free detection method is employed to detect rotavirus. The project is in collaboration with Dr. Mark Kuhlenschmidt and Theresa Kuhlenschmidt and funded by SRU Biosystems, Woburn, MA.
Reference
- “Detection of Rotavirus using Photonic Crystal Biosensor,” Maria Pineda, Leo L. Chan, Theresa Kulenschmidt, Mark Kulenschmidt, Brian T. Cunningham, Applied Environmental Microbiology, Submitted 19 February, 2008
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